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Antioxidant Activity of The Ancient Herb, Holy Basil in CCl4-Induced Liver Injury in Rats

Yuvaraj Ponnusam, Therasilin Louis, V Madhavachandran, Suresh Kumar, Neelam Thoprani, Michael R Hamblin, Shanmugamurthy Lakshmanan


An herbal preparation called “holy basil plus herbal powder†(HBPP) containing Ocimum santum, Withania somnifera, Pongamia pinnata, Plumbago indica, Emblica officinalis and Curcuma longa was investigated as an antioxidant and hepatoprotective agent. The antioxidant activity of HBPP was investigated in rats with liver injury induced by oral administration of carbon tetrachloride:olive oil (1:1). HBPP was administered orally at 500 mg/kg daily for 7 days before. HBPP exhibited statistically significant antioxidant activity, as shown by increased levels of glutathione peroxidase (GPX), glutathione S-transferase (GST), glutathione reductase (GRD), superoxide dismutase (SOD) and catalase (CAT) and decreased level of lipid peroxidation (LPO). HBPP performed equally well as silymarin, a well-established antioxidant preparation used to protect against liver injury.



Holy basil plus herbal powder; carbon tetrachloride; liver enzymes; antioxidant activity, hepatoprotection

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R.L. Zheng, S.A. Lesko and P.O.P. TS’O, Sci. Sin. Chin. Ed. 31 676-686 (1988)

Halliwell, B., Aeschbach, R., Loligger, J., Aruoma, O.I., 1995. The characteristic of antioxidants. Federal Chemical Toxicology 33, 601–617.

B. Halliwell, 1996. Annu. Rev. Nutr. 16 33-50.

B. Poeggeler, R.J. Retter, D.X. Tan, L.D. Chen and L.C. Manchester., 2000. Antioxidant Activity in Medicinal Herbs 735. J. Pineal Res. 14 151-168 (1993). Vol. 66, No. 8.

Necheles, T.F., Boles, T.A., Allen, D.M., 1968. Glutathione peroxidase assay method. Journal of Pediatrics 72, 319.

Habig, W.H., Pabst, M.J., Jocoby, W.B., 1974. The first enzyme step in mercaptouric

Dubler, R.E., Anderson, B.M., 1981. Simultaneous inactivation of the catalytic activities of yeast glutathione reductase by N-alkyl meleimimdes. Biochemica Biophysica Acta 659, 70.

Misra, H.P., Fridovich, I., 1972. The role of superoxide anion in the autooxidation of epinephrine and a simple assay for superoxide dismutase. Journal of Biological Chemistry 247, 3170–3185.

Bergmeyer, H.V., Gawehn, K., Grassik, M., 1994. Methods of enzymatic analysis. In: Bergmeyer, H.V., Chemie, V., Weinhein, S. (Eds.). Academic Press, New York, p. 348.

Ohkawa, H., Onishi, N., Yagi, K., 1979. Assay of lipid peroxidation in animal tissue by thiobarbituric acid reaction. Analytical Biochemistry 95, 351–354.

Doumas, B.T., Watson, W.A., Biggs, A.G., 1971. Estimation of total protein. Clinical Chemistry Acta 31, 87–96.

Eaton, J.W., 1991. Catalase, glutathione peroxidase and hydrogen peroxidase. Journal of Laboratory and Clinical Medicine 118, 3–4.

Floka, L., 1971. Glutathione peroxidase enzymologic and biological aspects. Klin Nochenschr 32, 49.

Jakoby, W.B., 1988. Detoxification, conjugation and hydrolysis in liver biology and pathology. In: Arias, I.M., Jakoby, W.B. (Eds.), Raven Press, New York, pp. 375–385.

Aniya, Y., Anders, M.W., 1985. Alteration of hepatic glutathione-Stransferase and release into serum after treatment with bromobenzene and carbon tetrachloride. Biochemical Pharmacology 39, 4239–4244.

Tappel, A.C., 1973. Lipid peroxidation damage to cell components. Federal Proceedings 32, 1870–1874.

Wilkinson, J.H., 1962. An introduction to diagnostic enzymology. In:Arnold, E. (Ed.). Academic press, London, p. 84.

Silybum marianum: Beyond Hepatoprotection. Bahmani M, Shirzad H, Rafieian S, Rafieian-Kopaei M.J Evid Based Complementary Altern Med. 2015 Feb 16. pii: 2156587215571116. [Epub ahead of print].



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