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Isolation, Production, Purification and Applications of Proteases from Pseudomonas aeruginosa

Shailima RD Vardhini, Mohammed Irfath


Proteases are the enzymes which have a host of applications. They are used in the industrial, pharmaceutical and food industry etc. Proteases contribute to two third of the global requirements. The bacterial sources form an interesting sources for the isolation of the protease enzymes. The production of this crucial enzyme has hence gained importance. In the present experiment the isolation, purification, production, media optimization of the protease enzyme was performed from the Pseudomonas aeruginosa.The optimum activity was found to be at pH 8 and temperature 30 oC. The effective Nitrogen source and Carbon source are found to be Peptone and sucrose with the highest specific activity of 41 IU/mL and 13 IU/mL respectively.


Protease, Industrial uses, Pseudomonas aeruginosa, casein media, Soil bacteria

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Sevinc N, Demirkan E: Production of Protease by Bacillus sp. N-40 isolated from soil and its enzymatic properties. J Biol Environ Sci 2011, 5:95-103.

Das G, Prasad MP: Isolation, purification & mass production of protease enzyme from Bacillus subtilis. Int. Res. J. Microbiol 2010, 1:26-31.

Gupta R, Beg Q, Lorenz P: Bacterial alkaline proteases: molecular approaches and industrial applications. Applied microbiology and biotechnology 2002, 59:15-32.

Anwar A, Saleemuddin M: Alkaline protease from Spilosoma obliqua: potential applications in bioâ€formulations. Biotechnology and applied biochemistry 2000, 31:85-89.

OÅ‚dak E, Trafny EA: Secretion of proteases by Pseudomonas aeruginosa biofilms exposed to ciprofloxacin. Antimicrobial agents and chemotherapy 2005, 49:3281-3288.

Fulekar MH, Geetha M, Sharma J: Bioremediation of Trichlorpyr Butoxyethyl Ester (TBEE) in bioreactor using adapted Pseudomonas aeruginosa in scale up process technique. Biology and Medicine 2009, 1:1-6.

Wilhelm S, Gdynia A, Tielen P, Rosenau F, Jaeger K-E: The autotransporter esterase EstA of Pseudomonas aeruginosa is required for rhamnolipid production, cell motility, and biofilm formation. Journal of bacteriology 2007, 189:6695-6703.

Jellouli K, Bayoudh A, Manni L, Agrebi R, Nasri M: Purification, biochemical and molecular characterization of a metalloprotease from Pseudomonas aeruginosa MN7 grown on shrimp wastes. Applied microbiology and biotechnology 2008, 79:989-999.

Izrael-Živković L, Gojgić-Cvijović G, Karadžić I: Isolation and partial characterization of protease from Pseudomonas aeruginosa ATCC 27853. Journal of the Serbian Chemical Society 2010, 75:1041-1052.

Glessner A, Smith RS, Iglewski BH, Robinson JB: Roles of Pseudomonas aeruginosa las andrhl Quorum-Sensing Systems in Control of Twitching Motility. Journal of bacteriology 1999, 181:1623-1629.

Wagner VE, Iglewski BH: P. aeruginosa biofilms in CF infection. Clinical reviews in allergy & immunology 2008, 35:124-134.

Galloway DR: Pseudomonas aeruginosa elastase and elastolysis revisited: recent developments. Molecular microbiology 1991, 5:2315-2321.

Kernacki KA, Hobden JA, Hazlett LD, Fridman R, Berk RS: In vivo bacterial protease production during Pseudomonas aeruginosa corneal infection. Investigative ophthalmology & visual science 1995, 36:1371-1378.

R.D.Shailima V: Isolation, Purification and media optimization of L-Asparaginas from Alcaligenes faecalis. International Journal of Pharma and Bio Sciences 2013, 4:1062.

Keay L, Wildi BS: Proteases of the genus Bacillus. I. Neutral proteases. Biotechnology and Bioengineering 1970, 12:179-212.

Najafi MF, Deobagkar D, Deobagkar D: Potential application of protease isolated from Pseudomonas aeruginosa PD100. Electronic journal of biotechnology 2005, 8:79-85.

Pastor MD, Lorda GS, Balatti A: Protease obtention using Bacillus subtilis 3411 and amaranth seed meal medium at different aeration rates. Brazilian Journal of Microbiology 2001, 32:6-9.

Ward OP: Proteolytic enzymes. 1985.



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