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Isolation, Production, Purification and Applications of Proteases from Pseudomonas aeruginosa

Shailima RD Vardhini, Mohammed Irfath

Abstract


Proteases are the enzymes which have a host of applications. They are used in the industrial, pharmaceutical and food industry etc. Proteases contribute to two third of the global requirements. The bacterial sources form an interesting sources for the isolation of the protease enzymes. The production of this crucial enzyme has hence gained importance. In the present experiment the isolation, purification, production, media optimization of the protease enzyme was performed from the Pseudomonas aeruginosa.The optimum activity was found to be at pH 8 and temperature 30 oC. The effective Nitrogen source and Carbon source are found to be Peptone and sucrose with the highest specific activity of 41 IU/mL and 13 IU/mL respectively.


Keywords


Protease, Industrial uses, Pseudomonas aeruginosa, casein media, Soil bacteria

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References


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DOI: http://dx.doi.org/10.14259%2Fbmc.v1i2.77

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