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Direct Shoot Regeneration And Agrobacterium Rhizogenes Mediated Rooting In Aegle Marmelos (L.) Corr .Serr. Nodal Explants

R Anitha, M K Lavanya

Abstract


Aegle marmelos  is one of sacred  medicinal tree in India, which has numerous medicinal properties. Micropropagation of A.marmelos is challenging and  direct regeneration of shoot is simpler and can be carried out with ease. Direct shoot regeneration was observed with MS medium supplemented with 0.5 mg/l IAA + 2  and 3 mg/l kinetin after 6 weeks of incubation. However, direct shoot regeneration was observed in nodal explants co-cultured  with Agrobacterium rhizogenes in 5,10 and 15 minutes exposure after 3 days .  Instead of hairy root formation, after 3weeks of incubation, tumors developed in explants exposed to 10 and 15min of  exposure. It is evident from this study that response to A.Rhizogenes differs  from plant to plant and the response is highly specific.

Keywords


Aegle marmelos, Direct Shoot induction, Agrobacterium rhizogenes, Root induction

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References


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DOI: http://dx.doi.org/10.14259%2Fpm.v2i2.31

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